Statistical-based approach in potential diagnostic application of urinary nucleosides in urogenital tract cancer.

Abstract

We aimed at evaluation the potential diagnostic role of urinary nucleosides in urogenital tract cancer. Concentrations of 12 nucleosides determined by LC-MS/MS were subjected to correlation, association and interaction analyses. We identified six pairs of nucleosides differently correlated in the group of patients and controls (p < 0.05). N-2-methylguanosine (odds ratio: 4.82; 95% CI: 1.78-12.93; p = 0.002) and N,N-dimethylguanosine (odds ratio: 5.45; 95% CI: 1.78-16.44; p = 0.003), were significantly associated with the disease risk (p-corrected = 0.004). Interaction between N-2-methylguanosine and adenosine (p-interaction = 0.019) suggested their multiplicative effect on the outcome. Urinary nucleosides, namely N,N-dimethylguanosine and N-2-methylguanosine may have the potential to serve as prognostic biomarkers. Gender-specific differences in urogenital tract cancer are likely to occur.