Statistical Study of Extract Keratin Protein from Waste Chicken Feather Based on Response Surface Methodology

Abstract

The feathers contain a significant amount of keratin protein, which is used in cosmetics, shampoos, hair treatment creams, and skin creams. Dissolving chicken feathers with reducing agent and then separating the protein from chemicals are the key steps involved. However, in order to enhance the amount of recovered keratin as much as possible, the best conditions for extracting keratin from chicken feathers are required. In this study, Response Surface Methodology (RSM) was used in order to simulate and optimize the operating parameters for extracting keratin from waste chicken feathers in order to increase the amount of keratin protein compared to previous studies. Dissolving chicken feathers using sodium sulphide as a reducing agent at various periods, temperatures, and concentrations is the first step in the fundamental technique. After the feathers have been dissolved with a reducing agent, the fluid is treated with an ammonium sulfate solution to precipitate the protein. As determined by a biuret test and UV-Vis analysis, the keratin protein had a maximum wavelength of 290 nm. Finally, the statistical optimization of the extraction conditions provided a better understanding of the reaction parameters. The optimum yield of keratin was achieved at 3.7 hours at 30.07°C with 0.05 M sodium sulfide.