Abstract
Effect of 19 different medium components on chitinase production by marine isolate Pantoea dispersa was studied by Plackett–Burman design. Each component was screened on the basis of ‘ p’ (probability) value, which was at 95% or above of confidence level. In the initial chitinase production medium containing five medium components chitinase production of 108 units/ml was obtained. In the Plackett–Burman method, significant factors influencing the chitinase production were screened. 4.21-fold increase in chitinase production was observed in 22nd medium of Plackett–Burman experimental design. Increase in production of endochitinase (3.95-fold) and chitobiase (2.31-fold) was observed.
Published Version
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