Statistical optimization of solid-state fermentation for the production of fungal inulinase from apple pomace

Abstract

The present work was designed to determine the suitability of apple pomace for inulinase production from Mucor circinelloides by solid-state fermentation (SSF). Central composite rotatable deign (CCRD) matrix (15 runs) of RSM was experimentally executed to investigate the influence of moisture, pH and fermentation time on fungal inulinase production from apple pomace by SSF. SSFs were performed at flask-level at 30 °C. Under optimized conditions viz., moisture 83.5%, pH 6.4 and fermentation time 5.8 days, maximum inulinase production was 411.3 IU/gds. Coefficient of variation (CV%) 0.17 indicates the lowest deviations between the experimental and predicted values. Multiple correlation coefficient (R2) was calculated to be 1.00 for inulinase production, which signifies the good correlation between the experimental and predicted results. Model's higher F-value 94,051.9 and “Lack-of-Fit” F-value 0.43 also verifies the authenticity of the quadratic model. The present article highlights the applicability of apple pomace as potent substrate for inulinase production by M. circinelloides.