Abstract
In this study we carried out optimization of bacterial cellulose (BC) production from Gluconoacetobacter hansenii NCIM 2529 using a statistical model. Optimization of medium components and physical parameters used for bacterial cellulose production in G. hansenii NCIM 2529 was performed by two-step optimization. Eight culture parameters were screened by the Plackett–Burman design and significant parameters were optimized by the response surface methodology using a central composite design. Through these two-phase experiments, optimization of BC production was carried out for high production. The maximum average productivity of BC with optimized medium was 0.71 g/100 mL upon fermentation in a shake flask. The average BC production was observed to increase by about 44 % using these statistical techniques of media optimization.
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