Abstract
Background: A sequential statistical strategy was used to optimize tannase production from Aspergillus tubingensis using tea stalks by solid-state fermentation. Results: First, through a Plackett-Burman design, inoculum size and incubation time (among seven tested variables) were identified as the most significant factors for tannase yield. The effects of significant variables were further evaluated through a single steepest ascend experiment and central composite design with response surface analysis. Under the optimal conditions, the experimental value of 84.24 U/gds closely matched the predicted value of 87.26 U/gds. Conclusions: The result of the statistical approach was 2.09 times higher than the basal medium (40.22 U/gds). The results were fitted onto a second-order polynomial model with a correlation coefficient (R2) of 0.9340, which implied an adequate credibility of the model. Normal 0 21 false false false ES-CL X-NONE X-NONE /* Style Definitions */ table.MsoNormalTable {mso-style-name:Tabla normal; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-parent:; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin-top:0cm; mso-para-margin-right:0cm; mso-para-margin-bottom:10.0pt; mso-para-margin-left:0cm; line-height:115%; mso-pagination:widow-orphan; font-size:11.0pt; font-family:Calibri,sans-serif; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-fareast-language:EN-US;}
Highlights
Tannin acyl hydrolase, normally known as tannase (EC 3.1.1.20) [1], catalyzes the hydrolysis of tannic acid by breaking its ester and depside bonds to release glucose and gallic acid [2]
A. tubingensis CICC 2651 was cultivated on potato dextrose agar (PDA) slants at 30°C for 4 d
During the course of our earlier studies, the maximum production of tannase by A. tubingensis CICC 265 was observed for 120 h, and the value only reached 40.22 units per gram of dry substrate (U/gds) using an initial TS medium
Summary
Normally known as tannase (EC 3.1.1.20) [1], catalyzes the hydrolysis of tannic acid by breaking its ester and depside bonds to release glucose and gallic acid [2]. The Plackett–Burman design (PBD) is widely used to determine the independent variables with significant effects on the response [14], whereas response surface methodology (RSM) has long been used to solve process optimization problems in the fields of chemical engineering and agro-biotechnology [15]. These methods can help to evaluate effective factors and simultaneously solve multivariate equations to study the interactions between variables, as well as to determine the optimum combination of variables for a desirable response. The results were fitted onto a second-order polynomial model with a correlation coefficient (R2) of 0.9340, which implied an adequate credibility of the model
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