Abstract
Background:Lipases have various commercial applications and microorganisms serve as a potential source of production.Objective:The aim of this paper was to study the effect of interactions among different production parameters on lipase yield ofAspergillus fumigatus.Method:Plackett Burman and Central Composite Design (CCD) were established by using Design Expert software 10.0.Results:In the present study, interactions were studied for six different variables such as inoculum size, pH, temperature, galactose concentration, peptone concentration and incubation time. In Plackett-Burman design, galactose concentration, peptone concentration, pH and incubation time were found to be important factors. Using the statistical approach, the optimum factors were found to be as: galactose concentration (1.5%), peptone concentration (1.8%), pH (10.0) and incubation time (72 h) at 45°C under response surface curves. Upon statistical analysis, the coefficient of determination (R2) obtained was 0.9318 which showed that the model was significant.Conclusion:The statistical tools used predicted the optimal conditions for the production of the lipase. The optimized parameters were galactose concentration 1.5%, peptone concentration 1.4%, temperature 45°C, pH 10.0 and incubation time of 72 h for obtaining a maximum lipase activity of 6.22 U/ml.
Highlights
Lipases (EC 3.1.1.3) are carboxylic ester hydrolases that catalyse the hydrolysis of triacylglycerol to glycerol and free fatty acids [1, 2]
Fungal isolate A6 was characterized by partial sequencing of 18s rRNA gene from Exceleris Genomics, Ahmedabad and identified as Aspergillus fumigatus KU891253.1
The optimization of lipase production by using the stepwise experimental design of fungus Asper-gillus flavus showed that the 100 g/L wheat bran was present in the culture medium and 20 g/L soybean oil as the inducer [10]
Summary
Lipases (EC 3.1.1.3) are carboxylic ester hydrolases that catalyse the hydrolysis of triacylglycerol to glycerol and free fatty acids [1, 2]. Emulsified esters of glycerine and long-chain fatty acids such as triolein and tripalmitin were split by true lipases. Lipases are increasingly becoming popular for various industrial applications because of their diverse enzymatic properties and substrate specificities [3, 4]. Accepted: March 10, 2019 fungi were potentially better for isolating different lipases with unique properties. Lipases are versatile enzymes which are capable of valuable transformations in the different areas like organic chemistry, biofuels, oleo-chemistry and pharmaceutical industries [8, 9]. Lipases have various commercial applications and microorganisms serve as a potential source of production
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