Abstract
A new stationary phase based on β-cyclodextrin and Poly(N-isopropylacrylamide) was prepared. The mass % of the polymer/100 g stationary phase was 23 %. Through investigating the effects of the acetonitrile (ACN) content of the mobile phase, the pH and the column temperature on the retention factor of polar and hydrophilic compounds in the ACN-rich mobile phase, it was found that the new stationary phase had the typical retention behavior of hydrophilic interaction liquid chromatography (HILIC). Moreover, the retention of solutes in the mobile phase exhibited “U-shaped” curves, which was an indication of HILIC/reversed-phase liquid chromatography (RPLC) mixed-mode retention behavior. In addition, the separation of some hydrophilic and different polar compounds was achieved by using the new material in HILIC and RPLC modes, respectively.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
