Abstract
The potential impacts of magnetic field exposures on brain development have raised public concern. In the present study, we aimed to investigate the biophysical effects of moderate-intensity (0.5 T, Tesla) static magnetic field (SMF) on mice neural progenitor cells (mNPCs). Our results showed that the SMF exposure increased the number of neurosphere formation and enhanced proliferative activity in mNPCs. In addition, our flow cytometry data demonstrated that the proportions of S phase and G2/M phase mNPCs were remarkably increased following 5 days of SMF exposure. Moreover, the level of a mitotic regulatory protein, cyclin B, was upregulated after SMF exposure. Furthermore, the mNPCs exposed to SMF exhibited a significant increase in Sox2 expression. When mNPCs were induced to differentiation, our immunofluorescence assay revealed that the percentage of neurons (Tuj-1-positive cells) but not astrocyte (s100β-positive cells) was significantly higher and displayed morphological complexity in the SMF group. Finally, our electrophysiological results demonstrated the mNPC-derived neurons from the SMF group showing a significantly increased in input resistance, which indicated more functional maturation. Based on these findings, it appears reasonable to suggest that SMF exposure could affect normal neurogenesis and promote neural lineage differentiation as well as neuronal maturation.
Highlights
In recent years, there has been a dramatic increment of research concerned with the influence of the magnetic field (MF) on biological functions, especially on the central nervous system
To determine whether the mice neural progenitor cells (mNPCs) proliferation was affected by moderate-intensity Static magnetic field (SMF) exposure, we first examined the cell proliferative capacity of mNPCs by neurosphere assay
Our results showed that exposure to SMF induced a significant increase in larger neurosphere numbers at days 5, 6, and 7 after plating (control/SMF: day 5, 18:06 ± 2:52/31:89 ± 1:94; day 6, 58:53 ± 4:42/86:44 ± 2:62; day 7, 50:33 ± 3:68/117:11 ± 8:04; p < 0:05, p < 0:01, Figure 1(d))
Summary
There has been a dramatic increment of research concerned with the influence of the magnetic field (MF) on biological functions, especially on the central nervous system. Extremely low-frequency electromagnetic field (ELF-EMF), emitted from electronic devices, has been well reported to exert multiple modulating effects on the nervous system such as hippocampal neurogenesis, synaptic plasticity, and neuronal apoptosis [1,2,3,4,5]. MF can either be static or time-varying (electromagnetic). Static magnetic field (SMF), in particular, is more user-friendly to apply in clinical therapy, because only simple magnet is used to generate SMF. SMF is constant, time-independent, and zero-frequency MF, which does not change in direction or magnitude over time. The therapeutic effect of different strength SMF in clinic has been demonstrated, there are contradictory
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
