State of the Art Cell Detection in Bone Marrow Whole Slide Images

Abstract

Context: Diseases of the hematopoietic system such as leukemia is diagnosed using bone marrow samples. The cell type distribution plays a major role but requires manual analysis of different cell types in microscopy images. Aims: Automated analysis of bone marrow samples requires detection and classification of different cell types. In this work, we propose and compare algorithms for cell localization, which is a key component in automated bone marrow analysis. Settings and Design: We research fully supervised detection architectures but also propose and evaluate several techniques utilizing weak annotations in a segmentation network. We further incorporate typical cell-like artifacts into our analysis. Whole slide microscopy images are acquired from the human bone marrow samples and annotated by expert hematologists. Subjects and Methods: We adapt and evaluate state-of-the-art detection networks. We further propose to utilize the popular U-Net for cell detection by applying suitable preprocessing steps to the annotations. Statistical Analysis Used: Evaluations are performed on a held-out dataset using multiple metrics based on the two different matching algorithms. Results: The results show that the detection of cells in hematopoietic images using state-of-the-art detection networks yields very accurate results. U-Net-based methods are able to slightly improve detection results using adequate preprocessing - despite artifacts and weak annotations. Conclusions: In this work, we propose, U-Net-based cell detection methods and compare with state-of-the-art detection methods for the localization of hematopoietic cells in high-resolution bone marrow images. We show that even with weak annotations and cell-like artifacts, cells can be localized with high precision.