STATE OF THE ANTIOXIDANT SYSTEM OF RAPANA VENOSA (VALENCIENNES, 1846) UNDER ACTION OF ANIONIC DETERGENT

Abstract

The antioxidant system in the ctenidium, hepatopancreas and nephridia of gastropod Rapana venosa under stress caused by an anionic detergent – sodium dodecyl sulfate were studied. The experimental animals were kept for 3, 24 and 72 hours in aquariums with sea water with addition of sodium dodecyl sulfate in concentration of 5 and 20, the maximum permissible concentration (MPC). We determined the total antioxidant activity (TAA) and the content of reduced glutathione (GSH). The degree of oxidative damage to biopolymers was evaluated by the level of malondialdehyde (MDA). It was shown that the MDA content under the action of sodium dodecyl sulfate increased only in ctenidium after 72 hours from the start of the experiment. The index remained unchanged in the hepatopancreas and nephridia. The level of GSH in the shellfish ctenidium increased in presence of sodium dodecyl sulfate in all periods of observation. The hepatopancreas GSH level was not changed, and in nephridia GSH level increased only after three days of observation. TAA decreased in the ctenidium within the first 3 hours of observation, and in hepatopancreas and nephridia only after 72 hours at the maximum concentration of the detergent. In the other terms of the experiment at all concentrations of sodium dodecyl sulfate TAA in the tested bodies remained at the control level. It is concluded that the antioxidant system is resistant to sodium dodecyl sulfate and has a great adaptive capacity.