Abstract
Incorporation of 14CO 2 into an insoluble product (starch) by isolated pea ( Pisum sativum) chloroplasts in relation to adenylates, inorganic pyrophosphate (PPi) and other compounds has been investigated in some detail. Formation of starch was favoured in the presence of PPi and catalytic amounts of adenylates (ADP or ATP) while 3-phosphoglyceric acid (PGA), ribose-5-phosphate (R5P), or fructose 1,6-biphosphate (F-B-P) had zero or very little effect. it is suggested that the effect of adenylates and PPi appears as a consequence of more rapid exchange of these compounds across the chloroplast envelope in pea than that observed in spinach chloroplasts.
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