Starch and Sucrose Synthesis in Phaseolus vulgaris as Affected by Light, CO2, and Abscisic Acid

Abstract

Phaseolus vulgaris L. leaves were subjected to various light, CO(2), and O(2) levels and abscisic acid, then given a 10 minute pulse of (14)CO(2) followed by a 5 minute chase with unlabeled CO(2). After the chase period, very little label remained in the ionic fractions (presumed to be mostly carbon reduction and carbon oxidation cycle intermediates and amino acids) except at low CO(2) partial pressure. Most label was found in the neutral, alcohol soluble fraction (presumed sucrose) or in the insoluble fraction digestable by amyloglucosidase. Sucrose formation was linearly related to assimilation rate (slope = 0.35). Starch formation increased linearly with assimilation rate (slope = 0.56) but did not occur if the assimilation rate was below 4 micromoles per square meter per second. Neither abscisic acid, nor high CO(2) in combination with low O(2) (thought to disrupt control of carbon metabolism) caused significant perturbations of the sucrose/starch formation ratio. These studies indicate that the pathways for starch and sucrose synthesis both are controlled by the rate of net CO(2) assimilation, with sucrose the preferred product at very low assimilation rates.