StarBright Dyes:-Build bigger better panels with superior dyes excitable by the violet and ultraviolet lasers.

Abstract

Abstract StarBright UltraViolet and StarBright Violet Dyes are the brightest ultraviolet and violet laser excitable fluorescent dyes specifically developed for flow cytometry. Their narrow excitation and emission mean that despite the increased brightness there is no loss of resolution due to excessive spillover and spreading. StarBright Dyes can be pre-mixed, fixed and have no requirement for a special buffer when multiplexing, providing researchers with the flexibility to build bigger, better panels. Here we demonstrate this flexibility by comparing freshly made and premixed antibody cocktails from Bio-Rad Antibodies to immunophenotype human peripheral blood. Cells were stained using common staining buffers with acquisition on the ZE5 Cell Analyzer to show how bigger multiplex panels can be built with ease.