Abstract
The adherence of Staphylococcal epidermidis to human amniotic membrane (HAM) was determined and compared with S. epidermidis to human, rabbit, and cat conjunctiva in vitro. Commercially available HAM and conjunctival specimens from humans, rabbits, and cats were exposed to S. epidermidis (3 x108 colony-forming units per milliliter) for 0, 5, 30 and 90 minutes, rinsed in sterile saline, then processed for light, scanning (SEM), and transmission (TEM) electron microscopy. Scanning electron microscopy (x2000) was used to quantify adherent bacteria mm2 of tissue. Bacterial adherence occurred with HAM and human, rabbit and cat conjunctiva and increased over time. Adherence to HAM was similar to that of human conjunctiva at 0, 5, and 30 minutes but greater at 90 minutes (1.57 x), although this difference was not statistically significant (P g.16). The authors conclude that caution must be taken to prevent possible ocular infection with the use of HAM for ocular surface transplantation. Michael D. Wagoner
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