Staphylococcus caledonicus sp. nov. and Staphylococcus canis sp. nov. isolated from healthy domestic dogs.

Logan L Newstead,K Varjonen,J Harris,Gavin K Paterson,T Nuttall,S Goodbrand

doi:10.1099/ijsem.0.004878

Abstract

Two strains, H8/1T and H16/1AT, of Gram-stain-positive, coagulase-negative staphylococci were isolated from separate healthy domestic dogs in Scotland. Both strains were genome sequenced and their inferred DNA–DNA hybridisation indicates that H8/1T and H16/1AT represent two novel species of the genus Staphylococcus. On the basis of the results of genome sequence analysis (genome blast distance phylogeny and single nucleotide polymorphism analysis) H8/1T is most closely related to Staphylococcus devriesei and H16/1AT most closely related to Staphylococcus felis. Also, average nucleotide identity distinguished H8/1T and H16/1AT from S. devriesei and S. felis as did minor phenotypic differences. On the basis of these results, it is proposed that H8/1T and H16/1AT represent novel species with the respective names Staphylococcus caledonicus and Staphylococcus canis. The type strain of S. caledonicus is H8/1T (=NCTC 14452T=CCUG 74789T). The type strain of S. canis is H16/1AT (=NCTC 14451T=CCUG 74790T)

Highlights

Two strains, H8/1T and H16/1AT, of Gram-s tain-p ositive, coagulase-negative staphylococci were isolated from separate healthy domestic dogs in Scotland
The draft genome sequence data from Staphylococcus caledonicus H8/1T is available under these accession numbers: BioSample, SAMN15065541; Sequence Read Archive, SRR11909362; and assembly, JABTXV000000000
The draft genome sequence data from Staphylococcus canis H16/1AT is available under these accession numbers: BioSample, SAMN14548534; Sequence Read Archive, SRR11498036; and assembly, JABANU000000000

Summary

Introduction

H8/1T and H16/1AT, of Gram-s tain-p ositive, coagulase-negative staphylococci were isolated from separate healthy domestic dogs in Scotland. The 16S rRNA gene sequences of Staphylococcus caledonicus H8/1T and Staphylococcus canis H16/1AT are available under accession numbers MT671558 and MT442037 respectively. The draft genome sequence data from Staphylococcus caledonicus H8/1T is available under these accession numbers: BioSample, SAMN15065541; Sequence Read Archive, SRR11909362; and assembly, JABTXV000000000.

Results

Conclusion