Staphylococcus aureus and Pseudomonas aeruginosa- Biofilm formation Methods

Abstract

For the detection of Biofilm formation method, total 60 clinical isolates viz. Staphylococcus aureus, Pseudomonas aeruginosa were used. Clinical isolates were identified as per standard microbiological procedure. Antibiotic susceptibility test of biofilm producing bacteria was performed by using the Kirby-Bauer disc diffusion technique. Biofilm detection was tested by Tube method (TM), Congo Red Agar method (CRA). These methods require the use of especially solid media-brain heart infusion broth supplemented with 5% sucrose and Congo red strain. Out of 60 isolates, CRA method detected 54 as high biofilm producer while by TM method, 50 isolates showed trong biofilm formation, and non biofilm producer were 10. According to the antibiotic susceptibility test, higher antibiotic resistance was observed in biofilm producing bacteria than non biofilm producers. Hence from the current investigation, CRA method is rapid, sensitive and reproducible. TM method gave moderate results. So CRA method is more suitable method for detection of biofilm formation as compared to the other method in the present investigation.