Stanniocalcin-1 promoting proliferation, migration and inhibition of apoptosis of 5637 cells in bladder cancer

Abstract

Objective To explore the expression of stanniocalcin-1 (STC-1) in bladder cancer and the effects of STC-1 on the biological behaviors of bladder cancer cell line 5637. Methods Cancer tissues and adjacent normal tissues were collected from 60 patients with bladder cancer. Reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blotting were applied to investigate the expression of STC-1 in bladder cancer tissues and paracancerous tissues. Cell counting kit-8 (CCK-8) assay, flow cytometry and Transwell assay were used to observe the effects of STC-1 protein on the proliferation, apoptosis and migration of 5637 cells. Results The relative expression level of STC-1 mRNA was (0.826±0.112) in bladder cancer tissues, which were over-expressed as compared with that in the paracancerous tissues (0.283±0.006) (P=0.000). The relative expression level of STC-1 protein was (0.795±0.107) in bladder tissues, which was up-regulated as compared with that in the paracancerous tissues (0.164±0.039) (P=0.000). In addition, although the level of STC-1 mRNA and protein increased with the higher clinical stages, there was no statistically significant difference. As compared with control group, STC-1 could promote the proliferation of 5637 cells. In addition, as compared with STC-1 monoclonal antibody group [(56.26±2.27)%] and blank control group [(53.43±2.04)%], the cell apoptosis ratio in STC-1 group [(18.48±0.87)%] decreased significantly (P=0.000). Transwell assay revealed that as compared with STC-1 monoclonal antibody group [(28±2) cells] and blank control group [(32±3) cells], the number of migrating cells in STC-1 group [(96±7)cells] was significantly increased (P=0.000). Conclusion Our results suggested that STC-1 might play a key role in the development of bladder cancer. Key words: Bladder cancer; Stanniocalcin-1; Proliferation; Apoptosis; Migration