Abstract
Exigencies as ethic plant raw material are part of the needs of modern phytotherapy. Micropropagation offers opportunities to obtain mass propagation of superior genotypes in short time. This study aimed to develop a protocol of direct and indirect organogenesis of Phyllanthus tenellus Roxb. Nodal segments from plantlets obtained by in vitro germination were subcultured in modified Murashige and Skoog medium added with different plant growth regulators: IAA (indole-3-acetic acid), IBA (indole-3-butyric acid), GA 3 (3-giberelic acid) and KIN (kinetin). The highest proliferation rate was obtained using the combinations: IBA, KIN + GA 3 (3.5 mg L -1 ) and IBA + KIN (2.4 mg L -1 ). Rooting was intensified after 40 days, reaching 100% for all media with indole-3-butyric acid. Addition of 2,4 dichlorophenoxyacetic acid (2,4D) provided the best results for production of friable calli. Acclimatization was 100% effective for plantlets cultured in control medium, with decrease in survival rate in grown plantlets from media added with growth regulators.
Highlights
Nowadays, there is an increasing interest in the use of medicinal plant, but insufficient information, quality and control of some phytotherapeutical products offered is evident, and may not bring the results expected by professionals and users (VICTÓRIO; LAGE, 2008)
The current study aimed to establish a system of standardized production of raw material of P. tenellus using different tissue culture techniques to guarantee the supply of ethic and qualified plant material properly identified
Media were supplemented with 3% sucrose, 1.48 μM thiamine-HCl, 2.43 μM pyridoxine, 4.1 μM nicotinic acid, 0.6 mM myoinositol, pH adjusted to 5.8 ± 0.1, sterilized in autoclave at 120 oC and 1.1 kgf cm-2 were employed
Summary
There is an increasing interest in the use of medicinal plant, but insufficient information, quality and control of some phytotherapeutical products offered is evident, and may not bring the results expected by professionals and users (VICTÓRIO; LAGE, 2008). Tissue culture techniques enable high efficiency and quick production of ethic and pathogens-free plants; besides provoke a considerable interest as a potential alternative to produce bioactive compounds. Is a member of Phyllanthaceae family, widely found in several places in Brazil, especially in Rio de Janeiro city (HOFFMANN et al, 2006). This species has been exploited as a source of herb medicines. The current study aimed to establish a system of standardized production of raw material of P. tenellus using different tissue culture techniques to guarantee the supply of ethic and qualified plant material properly identified
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