Standardized Edible Bird's Nest Extract Prevents UVB Irradiation-Mediated Oxidative Stress and Photoaging in the Skin.

Minhee Lee,Sangwon Eun,Seong-Hoo Park,Dakyung Kim,Wakana Yamada,Ok-Kyung Kim,Jeongmin Lee

doi:10.3390/antiox10091452

Abstract

We investigated whether standardized edible bird’s nest extract (BNE-PK) can prevent ultraviolet B (UVB) irradiation-mediated oxidative stress and photoaging in the skin using in vitro and in vivo models. BNE-PK increased skin hydration by hyaluronic acid synthesis and activation of ceramide synthase in UVB-irradiated hairless mice and HaCaT cells. Furthermore, BNE-PK suppressed melanogenesis by down-regulation of the cAMP/PKA/CREB/MITF/TRP-1/TRP-2/tyrosinase pathway in UVB-irradiated hairless mice and 3-isobutyl-1-methylxanthine (IBMX)-treated B16F10 cells. In UVB-irradiated hairless mice, BNE-PK attenuated the wrinkle formation-related JNK/c-FOS/c-Jun/MMP pathway and activated the TGF-βRI/SMAD3/pro-collagen type I pathway during UVB-mediated oxidative stress. Based on these findings, our data suggest that BNE-PK may potentially be used for the development of effective natural anti-photoaging functional foods for skin health.

Highlights

The skin is one of the important organs that performs biochemical and physical defense functions to protect the body’s internal structures from external threats such as ultraviolet (UV) radiation, harmful chemicals, and pathogens
The L-ascorbic acid, arbutin, and BNE-PK supplementation groups showed significant increase in the antioxidant activities of enzymes, including superoxide dismutase (SOD), catalase, and glutathione peroxidase (GPx), compared to those in the control group (p < 0.05) (Figure 2C–E). These results indicated that BNE-PK supplementation effectively suppressed ultraviolet B (UVB) irradiation-induced morphological and histopathological changes and oxidative stress in dorsal skin
We found that that UVB-irradiated hairless mice supplemented with L-ascorbic acid, arbutin, and BNE-PK showed a significant increase in hyaluronic acid synthesis-related factors, protein expression of hyaluronic acid synthase 2 (HAS2), and mRNA expression of

Summary

Introduction

The skin is one of the important organs that performs biochemical and physical defense functions to protect the body’s internal structures from external threats such as ultraviolet (UV) radiation, harmful chemicals, and pathogens. It is responsible for the barrier function to prevent water loss and the sensory function to detect external changes; it is susceptible to damage because it is directly exposed to the external environment [1,2]. The skin is composed of three layers: the epidermis, dermis, and subcutaneous fat layer, and each layer has its own structural and physiological functions. The dermis is mainly composed of matrix components such as collagen, elastin and extrafibrillar matrix, proteoglycans, and glycoproteins, which are made by fibroblasts that gives the skin strength and elasticity [4,5]

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Discussion

Conclusion