Standardized Analytical Procedure for Routine Testing of Amlodipine Besilate and Enalapril Maleate Simultaneously in In-vitro Samples

Abstract

>This study develops a standardized methodology for routine analysis of in-vitro samples of amlodipine besilate and enalapril maleate concurrently in immediate-release tablet dosage forms. UV-detection was performed at absorption maxima of 237.20 nm and 217.00 nm for amlodipine besilate and enalapril maleate, respectively; 6.8 pH phosphate buffer was used as dissolution media for 30 mintues, on USP II (Paddle) dissolution apparatus, at 50 RPM and 37.0 ± 0.5oC. The fallouts of the analysis were endorsed statistically. Results were satisfactory in the arrays of 1–19 μg mL-1 for amlodipine besilate and 1–9 μg mL-1 for enalapril maleate. The analysis results exhibited that the projected simultaneous method is modest, swift, precise, and accurate and used for the repetitive analysis of dissolution samples of amlodipine besilate and enalapril maleate in combined pharmaceutical dosage forms (tablets).