Standardization of surface sterilization protocol for <em>in vitro </em>mass multiplication of grape rootstock dog ridge (<em>Vitis champini </em>P.)

Abstract

To standardize surface sterilization protocol in grape Dog Ridge rootstock, two types of explants viz., axillary buds and shoot tips were subjected to surface sterilization with sodium hypochlorite (0.25% and 0.5%) for three different duration (5,10 & 15 minutes). Prior to surface sterilization, the explants were pretreated with Carbendazim (0.3% and 0.5%) for 30 min. Pre-treatment of the axillary buds with 0.5% Carbendazim for 30 minutes and surface sterilization with 70% ethanol for 30 seconds followed by 0.5% sodium hypochlorite for 10 minutes under laminar air flow chamber resulted in the highest survival rate (68.33%) with least contamination (15.00%) and mortality (16.67%). However, in shoot tips, the highest survival rate (56.67%) with least contamination (30.00%) and mortality (13.33%) was recorded by pre-treating with 0.3% Carbendazim for 30 minutes and surface sterilization with 70% ethanol for 30 seconds followed by 0.25% sodium hypochlorite for 10 minutes.