Standardization of Sucrose and 6-Benzyl Aminopurine for in vitro Micro Tuberization of Potato

Abstract

In vitro micro tuberization from regenerated plantlets of potato varieties was observed in Biotechnology Laboratory, Department of Biotechnology, Bangladesh Agricultural University. Tubers of potato cultivars Diamant and Cardinal were used as initial experimental materials for meristem culture. The experiment was consisted of three factors; variety (Diamant, Cardinal), sucrose concentration (3%, 6%, 9%, 12%) and 6-benzyl aminopurine (BAP) levels (2.5, 5.0, 7.5 mg L-1). As a whole, 24 treatments were laid out in complete randomized design with three replications. Among the varieties, Diamant required minimum days (6-17) for micro-tuber initiation, produced more number of micro-tubers (4.97) and produced more average weight of micro-tuber (120.39mg) but there had no significant difference. Among the sucrose levels, quickest (6-15 days) micro-tuber initiation, the highest number of micro-tubers vial-1 (5.06) and the highest average weight of micro-tuber (137.31 mg) were found in 9% sucrose level. For different BAP levels, quickest (6-15 days) micro-tuber initiation, the highest number of micro-tubers vial-1 (5.38) and the highest average weight of micro-tuber (126.31 mg) were found at 5.0 mg L-1. The best combination for minimum duration (6-8 days) of micro-tuber initiation, the highest number of micro-tubers vial-1 (6.00) and the highest average weight of micro-tuber (152.01 mg) was in Diamant with 9% sucrose at 5 mg L-1. Concomitantly, the lowest number micro-tubers vial-1 (2.00) and the lowest average weight of micro-tuber (89.98 mg) were found in Cardinal cultured with 3% sucrose media where at 7.5 mg L-1 BAP and at 2.5 mg L-1 BAP, respectively.