Standardization of silver nanoparticle synthesis: Photocatalytic application (immobilized with chitosan complex) with textile dyes and antibacterial activity against Staphylococcus aureus using banana pseudo stem

Abstract

The purpose of the study is to standardize the silver nanoparticle (BP-AgNPs) synthesis and its antibacterial activity and photocatalytic application with the selected dyes using the banana pseudo stem extract. “One-factor analysis (OFTA)” was carried out for the standardization of silver nanoparticle synthesis and nanoparticle-chitosan complex immobilization. The parameters were identified with plant quantity (20 g), silver nitrate concentration (1 mM), the ratio of plant extract and silver nitrate solution (2:8), pH (12), temperature (37 °C), dispersed light conditions, shaking conditions (120 rpm), and time (6 h) were analysed. The photocatalytic decolorization efficiency of the standardized BP-AgNPs (immobilized with chitosan complex) has shown 96.92% for methylene blue (10 ppm) at 3 h and 97.55% for safranin (100 ppm) at 15 h. The antibacterial activity for the synthesised BP-AgNPs was determined. MIC value of the BP-AgNPs was determined to be 15.62 μg. mL−1 for S. aureus. The synthesised BP-AgNPs treated with 0.5×, 1× and 2× MIC concentration (x = 15.62 μg. mL−1) showed decreased viable counts of S. aureus (99.6% at 2× concentration having viable count of 22.6 × 102 CFU. mL−1) at 24 h incubation when compared with the control culture. The structural characteristics of the BP-AgNPs were identified as spherical with SEM and the size was identified as 12.19 ± 1.62 nm with TEM and as 37.23 ± 17.89 nm with XRD. The parameters such as FTIR, Zeta potential, EDS further supports the nanoparticle synthesis with banana pseudostem extract. The current result suggested that, the silver nanoparticles (BP-AgNPs) synthesised using the extract of the banana pseudo stem could be used as an alternative source for dye decolorization and antibacterial activities.