Abstract
Objective: The main compounds in O. aristatus are rosmarinic acid, sinensetin, and eupatorin. Sinensetin and rosmarinic acid compounds have the potential as antiviral agents. The focus of this research is O. aristatus purple and white-purple varieties. This study aimed to determine the levels of three main secondary metabolites of O. aristatus, one of the specific standardizations.
 Methods: The standardization parameters to be tested were to determine the main compound levels by using thin-layer chromatography densitometry on two varieties of O. aristatus.
 Results: The highest value levels of sinensetin and rosmarinic acid in purple variety O. aristatus were 0.53 and 1.32% w/w, respectively. The highest level of eupatorin was 0.88% w/w in the ethanol extract of white-purple varieties of O. aristatus. The main secondary metabolites in the two varieties of O. aristatus were more significant in the leaves than in the stems. Meanwhile, the sinensetin and rosmarinic acid levels in the ethanol extract of leaves and stems of the purple variety O. aristatus were higher and significantly different than in the white-purple ones. However, the levels of eupatorin were higher and significantly (p<0.05) different in the white-purple variety compared to the purple variety.
 Conclusion: The purple variety is due to greater sinensetin and rosmarinic acid levels in the purple variety than in the white-purple ones.
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