Abstract
Hemoblobin A1c is the most important parameter for the monitoring of metabolic control of patients with diabetes mellitus. The purpose of this study was to adapt the Mono S method to a conventional HPLC system, allowing highly selective HbA1c determination without the acquisition of kits or the use of dedicated systems The results obtained were compared to the Tinaquant® immune turbidimetric method and the Bio-Rad Variant® chromatographic method. The developed method presented intra-study precision (C.V. %) of 1.39-3.65 and inter-study precision (C.V. %) of 2.80-3.02%. The determination coefficients among methods were: HPLC Mono S x Tinaquant®: r²: 0.9856 (n=60) and HPLC Mono S x HPLC Bio-Rad Variant®: r²: 0.9806 (n=16). A conversion equation between HPLC Mono S and Bio-Rad Variant® was calculated allowing yielding comparable and interchangeable values. The HPLC Mono-S is a precise, low-cost method which yields similar values to the Bio-Rad Variant® method on conventional HPLC equipment.
Highlights
Hemoglobin A1c (Hb A1c) is the most important laboratory parameter in monitoring metabolic control of patients with diabetes mellitus (Sacks et al, 2002).The quantity of HbA1c reflects the mean blood glucose concentration over the two or three preceding months and is an independent parameter of carbohydrate metabolism. (Miedema, 2004)
These values have since become widely used in clinical practice
We found no studies which employed the Mono S column for Hb A1c determination on HPLC systems, equipment that can be used for a broad range of other clinical analyses
Summary
Hemoglobin A1c (Hb A1c) is the most important laboratory parameter in monitoring metabolic control of patients with diabetes mellitus (Sacks et al, 2002).The quantity of HbA1c reflects the mean blood glucose concentration over the two or three preceding months and is an independent parameter of carbohydrate metabolism. (Miedema, 2004). Hemoglobin A1c (Hb A1c) is the most important laboratory parameter in monitoring metabolic control of patients with diabetes mellitus (Sacks et al, 2002). The quantity of HbA1c reflects the mean blood glucose concentration over the two or three preceding months and is an independent parameter of carbohydrate metabolism. Control and Complications Trial Research Group (DCCT). Proposed target levels of Hb A1c for diabetic control in order to minimize chronic complications characteristic of the disease such as retinopathy, nephropathy, and cardio-. These values have since become widely used in clinical practice
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