Abstract
The apo B quantitation by electroimmunoassay and immunonephelometry are compared for normal and pathological sera. The parameters affecting the accuracy and sensitivity of the assays, such as the selection of a suitable standard and the addition of a detergent, are discussed. Both assays were optimized by the addition of a lipoprotein-free serum to the low-density lipoprotein standard and dilution of the samples with a detergent. Under these conditions normal values for plasma lie in the range 80–90 mg/dl and the assay can be carried out on either plasma or serum from nonfasting or triglyceride-rich subjects with a variation coefficient of 6–7%.
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