Abstract
Standardization of every step in pre-analytic, analytic and post-analytic phases is crucial to achieve reproducible and reliable immunohistochemistry test results. This chapter delineates some critical points in pre-analytic, analytic and post-analytic phases, reiterates some important questions which may or may not have a consensus at this time, and updates the newly proposed guidelines on antibody validation from the College of American Pathologists Pathology and Laboratory Quality Center. Additionally, the chapter shares Geisinger’s experience with 1) testing/optimizing a new antibody and troubleshooting; 2) interpreting and reporting immunohistochemistry assay results; 3) improving and implementing a total immunohistochemistry quality management program; and 4) developing the concept of best practices in immunohistochemistry.
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