Abstract
Quality control standardizations of the various medicinal plants used in traditional medicine is becoming more important today in view of the commercialization of formulations based on these plants. An attempt at standardization of Cassia spectabilis leaf has been carried out with respect to authenticity, assay and chemical constituent analysis. The authentication involved many parameters, including gross morphology, microscopy of the leaves and functional group analysis by Fourier Transform Infrared (FTIR) spectroscopy. The assay part of standardization involved determination of the minimum inhibitory concentration (MIC) of the extract which could help assess the chemical effects and establish curative values. The MIC of the C. spectabilis leaf extracts was investigated using the Broth Dilution Method. The extracts showed a MIC value of 6.25 mg/mL, independent of the extraction time. The chemical constituent aspect of standardization involves quantification of the main chemical components in C. spectabilis. The GCMS method used for quantification of 2,4-(1H,3H)-pyrimidinedione in the extract was rapid, accurate, precise, linear (R2 = 0.8685), rugged and robust. Hence this method was suitable for quantification of this component in C. spectabilis. The standardization of C. spectabilis is needed to facilitate marketing of medicinal plants, with a view to promoting the export of valuable Malaysian Traditional Medicinal plants such as C. spectabilis.
Highlights
Herbal medicines are the therapeutic experiences of generations of practicing physicians of traditional medicine over hundreds of years and they are known to be oldest health care products that have been used by mankind all over the world to treat various types of ailments [1]
Since there are no reports in the literature regarding the standardization of C. spectabilis leaves, in the present investigation an attempt has been made to standardize them by using macroscopic and microscopic characteristics, authenticity, biological activity assay and chemical constituent analysis
C. spectabilis is a recognized source of useful chemicals for the pharmaceutical industries, but authenticity of specimens is important for these industries to avoid the acceptance of wrong plant materials for extracting actives, potentially resulting in a loss of billions of dollars
Summary
Herbal medicines are the therapeutic experiences of generations of practicing physicians of traditional medicine over hundreds of years and they are known to be oldest health care products that have been used by mankind all over the world to treat various types of ailments [1]. The body of information and controls necessary to produced materials of reasonable consistency This is achieved through minimizing the inherent variation of natural product composition through quality assurance practices applied to agricultural and manufacturing processes”[2]. In this paper we report an attempt for the standardization of Cassia spectabilis leaf with respect to authenticity, assay and chemical constituent analysis. Irwin et Barn (Leguminosae) is widely grown as an ornamental plant in tropical and subtropical areas, and has been commonly used in traditional medicine for many years. Since there are no reports in the literature regarding the standardization of C. spectabilis leaves, in the present investigation an attempt has been made to standardize them by using macroscopic and microscopic characteristics, authenticity, biological activity assay and chemical constituent analysis
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