Abstract
Arenavirus hemorrhagic fever is a severe emerging disease. Considering that the levels of antibodies against arenavirus in the Brazilian population are completely unknown, we have standardized an ELISA test for detecting IgG antibodies using a recombinant nucleoprotein from the Junin virus as the antigen. This protein was obtained by inserting the gene of the Junin virus nucleoprotein into the genome of Autographa californica nucleopolyhedrovirus, using the Bac-to-Bac baculovirus expression system. This recombinant baculovirus was used to infect S. frugiperda cells (SF9). The infection resulted in synthesis of high concentrations of recombinant protein. This protein was detected on 12.5% polyacrylamide gel and by means of Western blot. Using the standardized ELISA test, 343 samples from the population of Nova Xavantina were analyzed. We observed that 1.4% of the serum samples (five samples) presented antibody titers against arenavirus. These results show the population studied may present exposure to arenavirus infection.
Highlights
Arenavirus hemorrhagic fever is a severe emerging disease
In the present study, a recombinant nucleoprotein from JUNV was expressed and tested as the ELISA antigen for detecting arenavirus antibodies in a serological survey of the population of the municipality of Nova Xavantina, Brazil
The recombinant nucleoprotein (NP) of the Junin virus was expressed in the baculovirus system (Bac-to-Bac system, Invitrogen, USA)
Summary
Arenavirus hemorrhagic fever is a severe emerging disease. Methods: Considering that the levels of antibodies against arenavirus in the Brazilian population are completely unknown, we have standardized an ELISA test for detecting IgG antibodies using a recombinant nucleoprotein from the Junin virus as the antigen. In the present study, a recombinant nucleoprotein from JUNV was expressed and tested as the ELISA antigen for detecting arenavirus antibodies in a serological survey of the population of the municipality of Nova Xavantina, Brazil. Serum from 343 individuals living in the municipality of Nova Xavantina who presented fever with unidentified etiology had previously been analyzed in serological screening using hantavirus IgG-ELISA, and all these samples had
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