Abstract
Abstract A genotype independent media regime is optimized for clonal micro-propagation of selected cotton genotypes. The shoot tips of 7-days old seedlings of G. arboreum, G. herbaceum and G. hirstum var. S-12 and Sadori were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of benzyl-amino-purine (BAP) and kinetin (KIN) for shooting and Indol-butyric-acid (IBA) for rooting. Recoded observations revealed that 4.4 μM BAP and 5.75 μM KIN is the optimal combination of growth hormones to induce the highest number of shoots (5.92 shoots per explant) within minimum time (32.95 days) whereas rooting (3 roots per shoot) took minimally, 16-days at 3.75 μM IBA. The in vitro generated healthy plantlets were acclimatized for a month under high humidity with 91.75% survival rate. Additionally, cotton plantlets generated in vitro during this study were tested for the presence of CLCu-Virus (cotton leaf curl virus) by molecular diagnostics to be proved as virus free material.
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