Abstract
Objective: Identification of Chlorella species from the environment through 18s ribosomal RNA sequencing. This study was aimed to design primer targeting Chlorella and other closely related algal species targeting 18s ribosomal RNA, ITS1 region.
 Methods: Sanger sequencing was carried out for the identification of algae up to the genus and species level using an in-house designed primer and optimized PCR conditions.
 Results: Out of 2 algae samples identified phenotypically, one isolate identified as Chlorella vulgaris and other one identified as Chlorella sorokiniana based on the results of Basic Alignment Search Tool (BLAST).
 Conclusion: To conclude, this study provided primers with PCR conditions to characterize algal samples through molecular identification with 100% accuracy than the phenotypic method.
Highlights
Microalgae or microphytes are a ubiquitous group of fast-growing unicellular microscopic microphototrophs
Microalgae belong to phytoplankton can be isolated from freshwater and marine systems and they can survive in both the water column and sediment [1,2,3]
Two isolates of Blue-green algae were identified macroscopically, and both of them were amplified for the target 18s ribosomal RNA PCR
Summary
Microalgae or microphytes are a ubiquitous group of fast-growing unicellular microscopic microphototrophs. Valuable products from microalgae and its industrial commercialization today is still in its infancy and can be seen as a gateway to a multibillion-dollar industry. They represent a major untapped resource of genetic potential for valuable bioactive agents and fine biochemical [4]. Major research and development would entail the transition from a niche market to the widespread use of algal products as food commodities This will include improving existing strains through genetic engineering, and modifications or screening new species to the growth of microalgae with increased targeted metabolite production [5]. Microalgae achieve high potential as a feedstock for biofuel production due to their several advantages such as higher biomass productivity, lesser water demand, and no agricultural land requirement compared to other energy crops [14,15,16,17,18]
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