Standardisation of mycotoxin sampling procedures: an urgent necessity

Abstract

A mycotoxin sampling plan is defined by the mycotoxin test procedure (sample size, sample preparation method, and analytical method) and the accept/reject limit. Because of the variability associated with each step of the mycotoxin test procedure, the true mycotoxin concentration of a bulk lot cannot be determined with 100% certainty. As a result, some lots will be misclassified by the sampling program. Some good lots will be rejected by the sampling plan (seller’s risk or false positives) and some bad lots will be accepted by the sampling plan (buyer’s risk or false negatives). The magnitude of these risks is directly related to the magnitude of the variability associated with the mycotoxin test procedure. It is difficult for an exporter to have an effective control program when regulatory limits and sample designs differ greatly among trading countries. In order to facilitate trade and provide protection for the consumer, it would be desirable for all trading countries to have the same mycotoxin limits and sample plan. While standardization of sampling plans among trading nations is important, any standardised sampling plan must be designed to minimize both the seller’s and buyer’s risks to the lowest possible levels that resources will allow. Reducing the variability of the mycotoxin test procedure will reduce both the buyer’s and seller’s risks. It is important to understand the sources of error in the mycotoxin test procedure so the errors can be effectively reduced. The sampling step usually is the largest source of error due to the extreme mycotoxin distribution among kernels in the lot. As an example, sampling (5 kg), sample preparation (USDA subsampling mill and 250 g subsample), and analysis (TLC) accounted for 83%, 9%, and 8% of the total aflatoxin testing error, respectively, when testing raw shelled peanuts for aflatoxin. Examples are given to show how increasing sample size reduces sampling error; increasing the fineness of grind and using larger subsamples reduces sample preparation error, and increasing the number of aliquots analyzed and using improved technology (HPLC versus TLC) decreases analytical error. International organizations such as FAO/WHO have used scientific techniques to evaluate and design aflatoxin sampling plans for raw shelled peanuts traded in the export market.