Abstract
The use of dye-polysaccharide interactions in visualizing zones of polymer hydrolysis has been used to facilitate the detection of hemicellulolytic activity. Grams iodine was particularly effective in confirming the degradation of hemicellulosic pentosans and some α-glucans, whereas Congo Red and Trypan Blue interacted strongly with the β-d-glucans tested. Organisms, selectively isolated from garden compost, that produced iodinevisualized zones of hydrolysis all possessed xylanase activity; this enzyme was not detected in the isolates that failed to produce hydrolysis zones. Post-incubation staining with Grams iodine will therefore expedite the detection and unambiguous enumeration of hemicellulolytic isolates on enrichment culture.
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