Abstract
A sensitive staining method for alkali-labile phosphoproteins has been developed. As little as 0.2 nmol bound P/mm 2 can be detected. The procedure is based on alkaline hydrolysis, phosphate capture, and formation of an insoluble rhodamine B-phosphomolybdate complex. A further modification for the qualitative detection of alkaline phosphatase activity on polyacrylamide gels is proposed. During incubation, the released P i is precipitated as lead phosphate and subsequently stained with rhodamine B.
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