Abstract
The present study compared intracytoplasmic inclusion bodies in the locus ceruleus (LC) of mouse with protein bodies in the LC of human. Phosphotungstic acid-hematoxylin (PTAH) and the Mallory method were used as the anionic stains. The inclusion bodies and protein bodies, which stained with PTAH and the Mallory method in the brain, contain proteins that appear to belong to the same family. Although both inclusion and protein bodies were formed with the same arginine composition, their distribution in the brain was similar and similar physiological changes by morbidity were observed, and the fine structure of the inclusion bodies and protein bodies appeared to be different. The present findings suggest that mouse inclusion bodies and human protein bodies are different.
Highlights
In humans and non-human primates, the locus ceruleus (LC) corresponds to the region in the dorsolateral portion of the rostral mesencephalic and caudal mesencephalic tegmentum [1]
The positive-staining body (1.0 3.0 m in diameter) was recognized in the cytoplasm of most LC neurons with the modified Holmes method (Figure 1). In both Phosphotungstic acid-hematoxylin (PTAH) and Mallory’s trichrome-stained preparations of mouse LC, except for the neural nucleolus, the erythrocytes, the glial and endothelial nuclei and the myelin sheaths, little else seemed to stain in the tissue
Closer observation of the cytoplasm revealed that an inclusion body was often encountered in the cytoplasm of the mouse LC neurons
Summary
In humans and non-human primates, the locus ceruleus (LC) corresponds to the region in the dorsolateral portion of the rostral mesencephalic and caudal mesencephalic tegmentum [1] In the mouse, this nucleus is localized to the lateral part of the periventricular gray matter and is composed of closely packed, medium-sized nerve cells [2]. The existence of nucleolus-like inclusion bodies has been demonstrated in nerve cells of the central nervous system in normal mice and rats using electron microscopy (EM) [5,6]. These inclusion bodies are devoid of a limiting membrane and comprise aggregates of granular and/or filamentous materials.
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