Abstract
BackgroundAmylase is used commercially in food, textiles, sugar syrup, paper, and detergent industries. Bacteria and fungi remain a significant source of industrial enzymes. Pleurotus tuberregium is a macro-fungi that can exist as a fruiting body, sclerotium, mycelium, and spores. Some studies have been conducted on this fungus, with minimal studies on its enzyme activity (s) using the submerged fermentation technique. ResultsThe purified amylase has a specific activity of 5.26 U/mg, total activity of 189.20 U, maximally active at 70 °C, pH of 5, and retaining 100% of its activity at 30 oC for 4 min. P. tuberregium amylase showed optimal activity with plantain peel, followed by starch and pineapple peel (42, 30, and 29 μg/mL/min respectively). The presence of Ca2+, Mg2+, and Na+ ions in the reaction mixture activated the enzyme activity, but was slightly and moderately inhibited by KCl and Na2H2PO4 respectively. The crude enzyme effectively clarified juice, liquefied soluble cassava starch (with a release of appreciable glucose quantity), and partially de-stained white fabric. ConclusionsThe amylase obtained from the submerged fermentation of Pleurotus tuberregium has potential applications in food and detergent industries.
Highlights
Amylase is used commercially in food, textiles, sugar syrup, paper, and detergent industries
P. tuberregium is a versatile fungus that can utilize a variety of substrates
Effect of pH on amylase activity The purified amylase activity from P. tuber-regium was determined over a pH range of 3 to 10, but activity was detected from 3 to 8
Summary
Amylase is used commercially in food, textiles, sugar syrup, paper, and detergent industries. Two types are mainly recognized, exo- and endo-amylases. The former hydrolyses the non-reducing end of starch while the latter hydrolyses glycosidic linkages within the starch molecule [1]. Α-amylases are used in starch industry for liquefaction, where the partial hydrolysis of starch residues leads to the production of short chain dextrin. This lowers the viscosity of the starch solution and is further applied in the paper industry for hydrolyzing high molecular weight starch in coated paper. This improves the smoothness and strength of the paper. Α-amylases have found application in fuel alcohol production and textile industry
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