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Abstract
Eurycomanone is the most active compounds in the roots of Eurycoma longifolia and shown to have in vitro antiplasmodial activity. However, the stage of Plasmodium falciparum cycles which are sensitive to eurycomanone have not been investigated. This study was conducted to investigate stage specificity of eurycomanone at various stages of P. falciparum life cycles. Stage specificity of eurycomanone at various stages of P. falciparum was performed on P. falciparum culture in vitro. A total of 100 µL of solution containing P. falciparum at ring stage after synchronized with 1-2% parasitemia (hematocrit 3%) were included in 96 wells microcultures and then added 100 µL of solution containing eurycomanone with 6 various concentrations. The specificity of eurycomanone was evaluated microscopically by counting the percentage of each stage of P. falciparum after for 8, 16, 24, 32, 40, 48, 56, 64 and 72 h incubation time, compared with control without any compound. The results showed that eurycomanone can kill ring stage of P. falciparum and may inhibit the development of young schizont to mature schizont in vitro. However, it needs further investigations for the mechanism.
Highlights
Malaria is still health problem in the world
A part of our research program consists in the evaluation of the antiplasmodial activities of plants traditionally used in Indonesian regions to treat malaria, we have evaluated the antiplasmodial activity of some medicinal plants from South Kalimantan such as mahoni (Swientenia mahagoni Jack), brotowali (Tinospora tuberculata Beumee), mimba
The results showed that giving eurycomanone with all concentrations i.e., 10, 20, 40, 60, 80 and 100 ng mL−1 on P. falciparum showed that the difference percentage of Plasmodium stage started at 8 h incubation period
Summary
Malaria is still health problem in the world. An estimated 3.3 billion people in 97 countries and territories are at risk of being infected with malaria and developing disease and 1.2 billion are at high risk (WHO, 2014). The increasing resistance of Plasmodium falciparum strains to currently available anti-malarial has initiated numerous studies aimed at identifying new antimalarial agents. One of the strategies in search for new anti-malarial compounds is a research of active plant constituents. Medicinal plants have been used traditionally to treat of malaria in some countries in the world. Significant success was achieved with the new compounds extracted from plants like Qinghaosu (artemisinin) (Li and Rieckmann, 1992) and it has stimulated the search for new plant derived drugs
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