Stable sound decoding despite modulated sound representation in the auditory cortex

Abstract

The activity of neurons in the auditory cortex is driven by both sounds and non-sensory context. To investigate the neuronal correlates of non-sensory context, we trained head-fixed mice to perform a two-alternative-choice auditory task in which either reward or stimulus expectation (prior) was manipulated in blocks. Using two-photon calcium imaging to record populations of single neurons in the auditory cortex, we found that both stimulus and reward expectation modulated the activity of these neurons. A linear decoder trained on this population activity could decode stimuli as well or better than predicted by the animal's performance. Interestingly, the optimal decoder was stable even in the face of variable sensory representations. Neither the context nor the mouse's choice could be reliably decoded from the recorded neural activity. Our findings suggest that, in spite of modulation of auditory cortical activity by task priors, the auditory cortex does not represent sufficient information about these priors to exploit them optimally. Thus, the combination of rapidly changing sensory information with more slowly varying task information required for decisions in this task might be represented in brain regions other than the auditory cortex.