STABLE ISOTOPES IN ECOSYSTEM STUDIES

Abstract

The use of stable isotopes to solve biogeochemical problems in ecosystem analysis is increasing rapidly because stable isotope data can contribute both source-sink (tracer) and process information: The elements C, N, S, H, and all have more than one isotope, and isotopic compositions of natural materials can be measured with great precision with a mass spectrometer. Isotopic compositions change in predictable ways as elements cycle through the biosphere. These changes have been exploited by geochemists to understand the global elemental cycles. Ecologists have not until quite recently employed these techniques. The reasons for this are, first, that most ecologists do not have the background in chemistry and geochemistry to be fully aware of the possibilities for exploiting the natural variations in stable isotopic compositions, and second, that stable isotope ratio measurements require equipment not normally available to ecologists. This is unfortunate because some of the more intractable problems in ecology can be profitably addressed using stable isotope measurements. Stable isotopes are ideally suited to increase our understanding of element cycles in ecosystems. This review is written for ecologists who would like to learn more about how stable isotope analyses have been and can be used in ecosystem studies. We begin with an explanation of isotope terminology and fractionation, then summarize isotopic distributions in the C, N, and S biogeochemical cycles, and conclude with five case studies that show how stable isotope measurements can provide crucial information for ecosystem analysis. We restrict this review to studies of natural variations in C, N, and S isotopic abundances, cxcluding from consideration ~5N enrichment studies and hydrogen and oxygen isotope studies. Our focus on C, N, and S derives in part from our