Abstract
Synchronous measurement of brain neurotransmitters and blood drug metabolism by dual sites in vivo microdialysis can provide very important information for several neurological disorders such as post-stroke depression (PSD). In this work, d0/d3-6-N-methyl-rhodamine 6G-N-hydroxysuccinimidyl formate (d0/d3-MRSF) were firstly designed, synthesized and used as stable isotope labeling derivatization reagents for the determination of multiple neurotransmitters by ultra-high performance liquid chromatography-tandem mass spectrometry. The light d0-MRSF was used to label neurotransmitters in real samples and the heavy d3-MRSF was used to label standards, which served as internal standards for quantification to minimize matrix effect. The d0/d3-MRSF-neurotransmitter derivatives were easily adsorbed onto the surface of Fe3O4@graphene oxide. Magnetic dispersive solid phase extraction followed with stable isotope labeling derivatization was developed for efficient sample pretreatment. The permanent positively charged moiety of d0/d3-MRSF significantly enhanced the ionization efficiency of neurotransmitters. The comparison results indicated that the synthesized MRSF derivatization method can bring better detection sensitivity than the commercial dansyl chloride method about tens to hundreds of times on the same instrumentation. Specific and regular product ions of m/z 413.3 and 416.3, m/z 429.4 and 432.4 were observed for d0-and d3-MRSF labeled neurotransmitters, respectively. The limits of detection were in the range of 2.0–40.0 pM. The accuracies ranged from 92.3% to 108.2% with the intra- and inter-day precisions in the range of 2.2–12.8%. Synchronous and dynamic determination of concentrations changes of brain neurotransmitters and blood gastrodin of PSD rats was achieved. The results indicated that gastrodin had analogous regulating effect of brain neurotransmitter, which like the clinical medicine fluoxetine of PSD. Taken together, the developed method was demonstrated to be promising for sensitive, accurate and simultaneous determination of multiple neurotransmitters in trace biofluids.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
