Abstract
Human Papillomavirus (HPV) is a single major source in causing cervical cancer worldwide which is the most common cause of death in females. Around 85% of the cervical cancer cases occur in developing countries where existing vaccines are not available or unaffordable. Hence, alternative platforms are considered necessary for the development of cost-effective vaccines against HPV for their availability in developing countries. Plants offer unique advantages for vaccine production over fermenter-based systems. Pentameric capsomeres ofHPVhave been reported to generate elevated immune responses against HPV. In present study a modified HPV-16 L1 (L1 2xCysM) protein has been expressed as a fusion protein with glutathione-S-transferase (GST) into tobacco plastids. Seven transplastomic lines of Nicotiana benthamiana were generated using biolistic transformation technique. Site specific integration of the GST-L1 2xCysM and aadA genes was confirmed by PCR. Southern blot analysis verified homoplasmy of all transplastomic lines.AntigencaptureELISAwith theconformationspecific antibody Ritti01, showed protein expression as well as the retention of immunogenic epitopes of L1 protein. GST-L1 expressing tobacco plants were completely normal in their morphology. Taken together, this data contribute another step forward towards thedevelopmentof cost-effectiveplant-madevaccinesagainstHPV for resource poor countries.
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