Stable Colonization of Orally Administered Lactobacillus casei SY13 Alters the Gut Microbiota.

Yuanchun Yue,Shuwen Zhang,Xiaoyang Pang,Min Zhang,Jing Lu,Baoyu Yang,Khaled Nassar,Cai Zhang,Jiaping Lv,Xiaoxi Xu,Liu Liu

doi:10.1155/2020/5281639

Abstract

The gut microbiota plays an important role in intestinal health. Probiotics such as Lactobacillus are known to regulate gut microbes and prevent diseases. However, most of them are unable to colonize their stability in hosts' intestinal tracts. In this study, we investigated the ability of Lactobacillus casei SY13 (SY13) to colonize the intestinal tract of BALB/c mice, after its oral administration for a short-term (once for a day) and long-term (once daily for 27 days) duration. Furthermore, we also evaluated the influence of its administration on the gut microbial structure and diversity in mice. Male BALB/c mice were gavaged with 108 colony-forming units (CFU) of SY13, and TaqMan-MGB probe and Illumina MiSeq sequencing were performed to assess the colonization ability and bacterial community structure in the cecum contents. The results showed that long-term treatment with SY13 enhanced its ability to form a colony in the intestine tract in contrast to the short-term treatment group, whose colony was retained for only 3 days. Oral administration of SY13 also significantly enhanced the gut microbial diversity. Short-term treatment with SY13 (SSY13) elevated Firmicutes and diminished Bacteroidetes phyla compared with long-term treatment (LSY13) and controls. The findings laid the foundation for the study of probiotic colonization ability and improvement of microbiota for the prevention of gut diseases.

Highlights

Academic Editor: Osamu Handa e gut microbiota plays an important role in intestinal health
Male BALB/c mice were gavaged with 108 colony-forming units (CFU) of SY13, and TaqMan-MGB probe and Illumina MiSeq sequencing were performed to assess the colonization ability and bacterial community structure in the cecum contents. e results showed that long-term treatment with SY13 enhanced its ability to form a colony in the intestine tract in contrast to the short-term treatment group, whose colony was retained for only 3 days
The colonization ability of L. casei SY13 and the effects of oral administration its bacteria on gut microbiota alteration remain unclear. erefore, the aim of the present study was to evaluate the colonization ability of L. casei SY13 and explore its effects on gut microbial structure and diversity in mice treated by gavage once and 27 times. e results demonstrate that the stable colonization of L. casei SY13 is associated with dosage and lays a foundation for studying interactions between L. casei SY13 and other members of the gut microbiota

Summary

Introduction

Academic Editor: Osamu Handa e gut microbiota plays an important role in intestinal health. We investigated the ability of Lactobacillus casei SY13 (SY13) to colonize the intestinal tract of BALB/c mice, after its oral administration for a short-term (once for a day) and long-term (once daily for 27 days) duration. E balance, composition, and diversity of the gut microbiota are closely correlated with host metabolism, energy regulation, immune development, pathogen defense, and prevention of intestinal diseases such as inflammatory bowel disease (IBD) and colorectal cancer [2]. Erefore, the aim of the present study was to evaluate the colonization ability of L. casei SY13 and explore its effects on gut microbial structure and diversity in mice treated by gavage once (short-term) and 27 times (long-term). The colonization ability of L. casei SY13 and the effects of oral administration its bacteria on gut microbiota alteration remain unclear. erefore, the aim of the present study was to evaluate the colonization ability of L. casei SY13 and explore its effects on gut microbial structure and diversity in mice treated by gavage once (short-term) and 27 times (long-term). e results demonstrate that the stable colonization of L. casei SY13 is associated with dosage and lays a foundation for studying interactions between L. casei SY13 and other members of the gut microbiota

Objectives

Methods

Results