Abstract
A centrifugal force microscope (CFM) is a highly cost-effective and user-friendly method to apply force with a desktop centrifuge. Applying force in a biophysical assay allows for studying the mechanics of motor proteins or the folding pathway of DNA or proteins. However, in comparison to other methods—such as atomic force microscopy, optical tweezers, and magnetic tweezers—measurements of position in the CFM are limited. Position is measured by video microscopy which is affected by the shaking of the centrifuge when it begins to rotate and the unfocusing of the camera. To improve stability, we followed the original designs from Yang (Methods in Molecular Biology 2016) and then worked to enhance focus stability over longer periods of time; investigated sample illumination; and altered the counterbalance scheme to reduce mechanical noise. An enhanced stability would allow reliable collection of position data to be used to produce a force-extension curve of DNA.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
