Stabilization of Vesicular Stomatitis Virus L Polymerase Protein by P Protein Binding: A Small Deletion in the C-Terminal Domain of L Abrogates Binding

Abstract

We showed previously that cells expressing the vesicular stomatitis virus (VSV) L polymerase gene via the vaccinia–T7 RNA polymerase system accumulated 2- to 5-fold more L protein when the P protein was coexpressed (Canteret al.,1993,Virology194, 518–529). The results presented here provide an explanation for this phenomenon. Pulse–chase analysis revealed that L was unstable with a half-life of 3 to 6 hr if expressed in the absence of P protein, but was stable for at least 16 hr when coexpressed with a 10- to 15-fold molar excess of P. The P protein, in contrast, was stable under both conditions. Stabilization correlated with formation of a P:L polymerase complex evidenced both by coimmunoprecipitation and by glycerol gradient sedimentation analyses. A mutant L protein, lacking amino acids 1638 to 1673, was not stabilized by coexpression and showed no binding to P protein. Its anomalous sedimentation, however, suggested misfolding and/or aggregation as the cause for the failure to bind P. Transcription reconstitutionin vitro,using extracts from cells expressing excess of P over L protein, strongly depended on coexpression of the proteins for optimal activity. We propose that the coexpression dependence for polymerase reconstitution documented here for VSV, as well as that reported previously for the Sendai paramyxovirus, reflects the protective effect of P protein on L protein stability.