Abstract
A stabilized reagent for the determination of total polyphenols by the Prussian blue method was developed. H3PO4 complexed with excess Fe3+ in the mixture, stopping the reaction. Gum acacia, a protective colloid, prevented precipitation by impeding the coalescence of the colloidal particles formed. Efficiency of chelating agents tried was H3PO4 (A) Na4EDTA (B) sodium hexametaphosphate (C). Effectiveness of protective colloids was gum acacia (D) = Reten (an anionic polyacrylamide) (E) Methocel 60 HG (F) phosphomannan 2448 (G) starch (H) polysaccharide B 1828 (I). Both the chelating agent and the protective colloid must be added to effect stabilization. Effectiveness of combinations was (A + D or E) (B + D) (A + F) (A + G) all other combinations. (A + D) prevented change in color density and prevented precipitation for up to 60 h with catechin as the polyphenol
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