Stabilization of sunflower oil by pennyroyal (Mentha piperita) extracts during accelerated storage

Abstract

ABSTRACTThe aim of this study was to examine the antioxidant activity of pennyroyal (Mentha piperita) extracts of sunflower oil as a replacement for synthetic antioxidant. This study was performed in two stages. In the current study, it was proven that the examined pennyroyal was in all ways similar to Mentha piperita sequence and its blast performance at the National Center for Biotechnology Information (NCBI). The anti-oxidation activities of Mentha piperita extracts were identified using 2,2-diphenyl-1-picrylhydrazyl and total phenolic content. The protective effect of extracts on the stabilization of sunflower oil was tested and compared with tertiary butyl hydroquinone by measuring peroxide values (y1), p-anisidine (y2), and rancimat value (y3) during storage for 4 weeks at 60°C (Schaal test).The results showed that the antioxidant activity of Mentha piperita extracts (500 ppm) was equal with tertiary butyl hydroquinone effect (100 ppm) at 60°C. In the next step, the sunflower oil containing Mentha piperita extracts (500 ppm) and tertiary butyl hydroquinone (100 ppm) as two blocks were tested to determinate the optimum condition of both high temperature (x1:180–220°C) and time (x2: 5–15 min) variables on oxidative stability. The optimum conditions were optimized using response surface methodology. The analysis of variance result showed that temperature and time had significant effects on peroxide, p-anisidine, and rancimat values (p < 0.01). The optimal condition for the examined parameters was related to a sunflower oil treatment containing Mentha piperita extracts (500 ppm) at 180°C and 5 min (with the desirability of 92.1%).