Abstract
Abstract 1. 1. Stability to frozen storage of the steroid-binding proteins of several species of fish for the principal androgens and corticosteroids was investigated. 2. 2. The proteins that bind steroids were quite stable in all species during storage at -40°C. 3. 3. There was a loss in binding of steroids by cod and haddock plasma but not by salmon plasma stored at -17°C. 4. 4. The proteins that bind corticosteroids were less stable than those that bind androgens. 5. 5. Saturation of plasma with nitrogen did not stabilize the steroid-binding proteins nor did storage in glass as contrasted with plastic bottles. 6. 6. Since the stability of fish plasma proteins to frozen storage is not the same for all species, it is suggested that steroid-binding studies are best carried out on fresh plasma when the stability of the protein is not known.
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