Abstract
Mouse-rat hybrid somatic cells were isolated by fusion of chloramphenicol-sensitive (CAP s) mouse fibroblast cells with hypoxanthine-guanine-phosphoribosyltransferase-deficient (HGPRT −) and CAP-resistant (CAP r) rat myoblast cells and selected with hypoxanthine-aminopterin-thymidine (HAT) and CAP. Restriction endonuclease cleavage patterns showed that both mouse and rat mitochondrial DNAs (mtDNAs) were present in the hybrid cells and that the amount of rat mtDNA was one-quarter that of mouse mtDNA, even after cultivation for 3 months in the presence of CAP. Nuclear ribosomal RNA (rRNA) genes of mouse and rat were shown to be expressed stably in the hybrid cells by homochromatography fingerprinting of RNase T1 digests. The genetic compatibility between mouse and rat chromosomes in the mouse-rat hybrid cells assures retention of both parental chromosomes, and this may be responsible for the expression of both parental rRNA genes, and for the retention of both parental mtDNAs in the hybrid cells.
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