Abstract
An investigation was made of livers from 5 dogs receiving 3.6 to 14 μg (specific activity = 1 mc/mg) of vitamin B12-Co60 intravenously. The vitamin B12-Co60, was retain ed by the livers as an intact cobalamin, probably as cyanocobalamin. This was demonstrated by the method of reverse isotope dilution. Direct isotope dilution indicated an average total cobalamin concentration of 0.36 μg/g of dog liver. The authenticity of retained vitamin B12-Co60, introduced by injection, as an isotope dilution tracer was demonstrated by simultaneous analysis with vitamin B12 Co57. Degradation of vitamin B12 to ionic cobalt was negligible.
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Schedule a callMore From: Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)
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